Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Full article: Paracetamol: overdose induced oxidative stress toxicity, metabolism, and protective effects of various compounds in vivo and in vitro g glutamyl cycle. Depletion of glutathione by acetaminophen leads to Download Scientific Diagram Paracetamol (acetaminophen) poisoning: The early years Prescott 2024 British Journal of Clinical Pharmacology Wiley Online Library View of Acetaminophen, the missing anion gap in the patient with metabolic acidosis The Southwest journal of medicine