TYLENOL [Acetaminophen] metabolism A lot of GLUTATHIONE is needed to deal with the Oxidative Stress generated by the CYP 450 metabolism of Tylenol. When people go to the ER for Tylenol Overdose Acetaminophen's Role in Autism and ADHD: A Mitochondrial Perspective Liver injury induced by paracetamol and challenges associated with intentional and unintentional use Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. paracetmol poisoning 160215161539 (1).pdf